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Detection of bacterial endosymbionts in freshwater crustaceans: the applicability of non-degenerate primers to amplify the bacterial 16S rRNA gene

Bacterial endosymbionts of aquatic invertebrates remain poorly studied. This is at least partly due to a lack of suitable techniques and primers for their identification. We designed a pair of non-degenerate primers which enabled us to amplify a fragment of ca. 500 bp of the 16S rRNA gene from vario...

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Detalles Bibliográficos
Autores principales: Mioduchowska, Monika, Czyż, Michał Jan, Gołdyn, Bartłomiej, Kilikowska, Adrianna, Namiotko, Tadeusz, Pinceel, Tom, Łaciak, Małgorzata, Sell, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296333/
https://www.ncbi.nlm.nih.gov/pubmed/30581663
http://dx.doi.org/10.7717/peerj.6039