Optimization of expression JTAT protein with emphasis on transformation efficiency and IPTG concentration

One of small accessory genes between pol and env is tat gene encoding TAT protein. This research was aimed to optimize the expression of Jembrana TAT (JTAT) protein with preparing Escherichia coli (E. coli) in advance using adopted methods of M1 (MgCl(2) + CaCl(2)) and M2 (CaCl(2) + Glycerol). The b...

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Detalles Bibliográficos
Autores principales: Margawati, Endang Tri, Fuad, Asrul Muhamad, Indriawati, Ridwan, Muhamad, Volkandari, Slamet Diah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Scientific Research and Technology, Egypt 2017
Materias:
Others (In silico & Biochemistry)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296586/
https://www.ncbi.nlm.nih.gov/pubmed/30647694
http://dx.doi.org/10.1016/j.jgeb.2017.06.009

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296586/
https://www.ncbi.nlm.nih.gov/pubmed/30647694
http://dx.doi.org/10.1016/j.jgeb.2017.06.009

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