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Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring

Early life nutritional imbalances are risk factors for metabolic dysfunctions in adulthood, but the long term effects of perinatal exposure to high versus low protein diets are not completely understood. We exposed C57BL/6J offspring to a high protein/low carbohydrate (HP/LC) or low protein/high car...

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Autores principales: Martin, Lisa J., Meng, Qingying, Blencowe, Montgomery, Lagarrigue, Sandrine, Xiao, Sheila, Pan, Calvin, Wier, Julian, Temple, William C., Devaskar, Sherin U., Lusis, Aldons J., Yang, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6297185/
https://www.ncbi.nlm.nih.gov/pubmed/30619467
http://dx.doi.org/10.3389/fgene.2018.00642
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author Martin, Lisa J.
Meng, Qingying
Blencowe, Montgomery
Lagarrigue, Sandrine
Xiao, Sheila
Pan, Calvin
Wier, Julian
Temple, William C.
Devaskar, Sherin U.
Lusis, Aldons J.
Yang, Xia
author_facet Martin, Lisa J.
Meng, Qingying
Blencowe, Montgomery
Lagarrigue, Sandrine
Xiao, Sheila
Pan, Calvin
Wier, Julian
Temple, William C.
Devaskar, Sherin U.
Lusis, Aldons J.
Yang, Xia
author_sort Martin, Lisa J.
collection PubMed
description Early life nutritional imbalances are risk factors for metabolic dysfunctions in adulthood, but the long term effects of perinatal exposure to high versus low protein diets are not completely understood. We exposed C57BL/6J offspring to a high protein/low carbohydrate (HP/LC) or low protein/high carbohydrate (LP/HC) diet during gestation and lactation, and measured metabolic phenotypes between birth and 10 months of age in male offspring. Perinatal HP/LC and LP/HC exposures resulted in a decreased ability to clear glucose in the offspring, with reduced baseline insulin and glucose concentrations in the LP/HC group and a reduced insulin response post-glucose challenge in the HP/LC group. The LP/HC diet group also showed reduced birth and weanling weights, whereas the HP/LC offspring displayed increased weanling weight with increased adiposity beyond 5 months of age. Gene expression profiling of hypothalamus and liver revealed alterations in diverse molecular pathways by both diets. Specifically, hypothalamic transcriptome and pathway analyses demonstrated perturbations of MAPK and hedgehog signaling, processes associated with neural restructuring and transmission, and phosphate metabolism by perinatal protein imbalances. Liver transcriptomics revealed changes in purine and phosphate metabolism, hedgehog signaling, and circadian rhythm pathways. Our results indicate maternal protein imbalances perturbing molecular pathways in central and peripheral metabolic tissues, thereby predisposing the male offspring to metabolic dysfunctions.
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spelling pubmed-62971852019-01-07 Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring Martin, Lisa J. Meng, Qingying Blencowe, Montgomery Lagarrigue, Sandrine Xiao, Sheila Pan, Calvin Wier, Julian Temple, William C. Devaskar, Sherin U. Lusis, Aldons J. Yang, Xia Front Genet Genetics Early life nutritional imbalances are risk factors for metabolic dysfunctions in adulthood, but the long term effects of perinatal exposure to high versus low protein diets are not completely understood. We exposed C57BL/6J offspring to a high protein/low carbohydrate (HP/LC) or low protein/high carbohydrate (LP/HC) diet during gestation and lactation, and measured metabolic phenotypes between birth and 10 months of age in male offspring. Perinatal HP/LC and LP/HC exposures resulted in a decreased ability to clear glucose in the offspring, with reduced baseline insulin and glucose concentrations in the LP/HC group and a reduced insulin response post-glucose challenge in the HP/LC group. The LP/HC diet group also showed reduced birth and weanling weights, whereas the HP/LC offspring displayed increased weanling weight with increased adiposity beyond 5 months of age. Gene expression profiling of hypothalamus and liver revealed alterations in diverse molecular pathways by both diets. Specifically, hypothalamic transcriptome and pathway analyses demonstrated perturbations of MAPK and hedgehog signaling, processes associated with neural restructuring and transmission, and phosphate metabolism by perinatal protein imbalances. Liver transcriptomics revealed changes in purine and phosphate metabolism, hedgehog signaling, and circadian rhythm pathways. Our results indicate maternal protein imbalances perturbing molecular pathways in central and peripheral metabolic tissues, thereby predisposing the male offspring to metabolic dysfunctions. Frontiers Media S.A. 2018-12-11 /pmc/articles/PMC6297185/ /pubmed/30619467 http://dx.doi.org/10.3389/fgene.2018.00642 Text en Copyright © 2018 Martin, Meng, Blencowe, Lagarrigue, Xiao, Pan, Wier, Temple, Devaskar, Lusis and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Martin, Lisa J.
Meng, Qingying
Blencowe, Montgomery
Lagarrigue, Sandrine
Xiao, Sheila
Pan, Calvin
Wier, Julian
Temple, William C.
Devaskar, Sherin U.
Lusis, Aldons J.
Yang, Xia
Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring
title Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring
title_full Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring
title_fullStr Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring
title_full_unstemmed Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring
title_short Maternal High-Protein and Low-Protein Diets Perturb Hypothalamus and Liver Transcriptome and Metabolic Homeostasis in Adult Mouse Offspring
title_sort maternal high-protein and low-protein diets perturb hypothalamus and liver transcriptome and metabolic homeostasis in adult mouse offspring
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6297185/
https://www.ncbi.nlm.nih.gov/pubmed/30619467
http://dx.doi.org/10.3389/fgene.2018.00642
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