ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous recombination...

Descripción completa

Detalles Bibliográficos
Autores principales: Murphy, Kenan C., Nelson, Samantha J., Nambi, Subhalaxmi, Papavinasasundaram, Kadamba, Baer, Christina E., Sassetti, Christopher M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299477/
https://www.ncbi.nlm.nih.gov/pubmed/30538179
http://dx.doi.org/10.1128/mBio.01467-18

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299477/
https://www.ncbi.nlm.nih.gov/pubmed/30538179
http://dx.doi.org/10.1128/mBio.01467-18

Ejemplares similares