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Quantitation of pulmonary fungal burden in Paracoccidioides brasiliensis-infected mice by real-time PCR
Although colony-forming unit (CFU) counting is widely used to quantify fungal load in tissue from animal experimentally infected with Paracoccidioides brasiliensis, several technical disadvantages have been described. Here we developed highly accurate quantitative PCR (qPCR) assays to determine the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto de Medicina Tropical
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300791/ https://www.ncbi.nlm.nih.gov/pubmed/30570075 http://dx.doi.org/10.1590/S1678-9946201961002 |