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Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa

A gene named zenc, encoding a zearalenone lactonase from Neurospora crassa, was over-expressed in Pichia pastoris. The zenc gene is 888-bp in length, encoding a 295-residue polypeptide. Purified ZENC has maximal activity at pH 8.0 and 45 °C, and is highly stable at pH 6.0–8.0 for 1 h at 37 °C. The a...

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Detalles Bibliográficos
Autores principales: Bi, Ke, Zhang, Wen, Xiao, Zhizhuang, Zhang, Dawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301899/
https://www.ncbi.nlm.nih.gov/pubmed/30570697
http://dx.doi.org/10.1186/s13568-018-0723-z