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Conventional versus real‐time quantitative PCR for rare species detection

Detection of species in nature at very low abundance requires innovative methods. Conventional PCR (cPCR) and real‐time quantitative PCR (qPCR) are two widely used approaches employed in environmental DNA (eDNA) detection, though lack of a comprehensive comparison of them impedes method selection. H...

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Detalles Bibliográficos
Autores principales: Xia, Zhiqiang, Johansson, Mattias L., Gao, Yangchun, Zhang, Lei, Haffner, Gordon Douglas, MacIsaac, Hugh J., Zhan, Aibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303721/
https://www.ncbi.nlm.nih.gov/pubmed/30598777
http://dx.doi.org/10.1002/ece3.4636