A mixing heteroduplex mobility assay (mHMA) to genotype homozygous mutants with small indels generated by CRISPR-Cas9 nucleases

Abstract The development of gene editing technologies, especially the CRISPR-Cas9 system, has been pivotal for understanding the functional role of proteins. Rapid and efficient genotyping methods are necessary to screen for generated mutations and streamline the isolation of homozygotes. CRISPR-Cas...

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Detalles Bibliográficos
Autores principales: Foster, Samantha D., Glover, Sarah R., Turner, Ashley N., Chatti, Kiranam, Challa, Anil K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305891/
https://www.ncbi.nlm.nih.gov/pubmed/30591915
http://dx.doi.org/10.1016/j.mex.2018.11.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305891/
https://www.ncbi.nlm.nih.gov/pubmed/30591915
http://dx.doi.org/10.1016/j.mex.2018.11.017

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