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Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes
The sarcomere is the contractile unit within cardiomyocytes driving heart muscle contraction. We sought to test the mechanisms regulating actin and myosin filament assembly during sarcomere formation. Therefore, we developed an assay using human cardiomyocytes to monitor sarcomere assembly. We repor...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307863/ https://www.ncbi.nlm.nih.gov/pubmed/30540249 http://dx.doi.org/10.7554/eLife.42144 |
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author | Fenix, Aidan M Neininger, Abigail C Taneja, Nilay Hyde, Karren Visetsouk, Mike R Garde, Ryan J Liu, Baohong Nixon, Benjamin R Manalo, Annabelle E Becker, Jason R Crawley, Scott W Bader, David M Tyska, Matthew J Liu, Qi Gutzman, Jennifer H Burnette, Dylan T |
author_facet | Fenix, Aidan M Neininger, Abigail C Taneja, Nilay Hyde, Karren Visetsouk, Mike R Garde, Ryan J Liu, Baohong Nixon, Benjamin R Manalo, Annabelle E Becker, Jason R Crawley, Scott W Bader, David M Tyska, Matthew J Liu, Qi Gutzman, Jennifer H Burnette, Dylan T |
author_sort | Fenix, Aidan M |
collection | PubMed |
description | The sarcomere is the contractile unit within cardiomyocytes driving heart muscle contraction. We sought to test the mechanisms regulating actin and myosin filament assembly during sarcomere formation. Therefore, we developed an assay using human cardiomyocytes to monitor sarcomere assembly. We report a population of muscle stress fibers, similar to actin arcs in non-muscle cells, which are essential sarcomere precursors. We show sarcomeric actin filaments arise directly from muscle stress fibers. This requires formins (e.g., FHOD3), non-muscle myosin IIA and non-muscle myosin IIB. Furthermore, we show short cardiac myosin II filaments grow to form ~1.5 μm long filaments that then ‘stitch’ together to form the stack of filaments at the core of the sarcomere (i.e., the A-band). A-band assembly is dependent on the proper organization of actin filaments and, as such, is also dependent on FHOD3 and myosin IIB. We use this experimental paradigm to present evidence for a unifying model of sarcomere assembly. |
format | Online Article Text |
id | pubmed-6307863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63078632019-01-02 Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes Fenix, Aidan M Neininger, Abigail C Taneja, Nilay Hyde, Karren Visetsouk, Mike R Garde, Ryan J Liu, Baohong Nixon, Benjamin R Manalo, Annabelle E Becker, Jason R Crawley, Scott W Bader, David M Tyska, Matthew J Liu, Qi Gutzman, Jennifer H Burnette, Dylan T eLife Cell Biology The sarcomere is the contractile unit within cardiomyocytes driving heart muscle contraction. We sought to test the mechanisms regulating actin and myosin filament assembly during sarcomere formation. Therefore, we developed an assay using human cardiomyocytes to monitor sarcomere assembly. We report a population of muscle stress fibers, similar to actin arcs in non-muscle cells, which are essential sarcomere precursors. We show sarcomeric actin filaments arise directly from muscle stress fibers. This requires formins (e.g., FHOD3), non-muscle myosin IIA and non-muscle myosin IIB. Furthermore, we show short cardiac myosin II filaments grow to form ~1.5 μm long filaments that then ‘stitch’ together to form the stack of filaments at the core of the sarcomere (i.e., the A-band). A-band assembly is dependent on the proper organization of actin filaments and, as such, is also dependent on FHOD3 and myosin IIB. We use this experimental paradigm to present evidence for a unifying model of sarcomere assembly. eLife Sciences Publications, Ltd 2018-12-12 /pmc/articles/PMC6307863/ /pubmed/30540249 http://dx.doi.org/10.7554/eLife.42144 Text en © 2018, Fenix et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Fenix, Aidan M Neininger, Abigail C Taneja, Nilay Hyde, Karren Visetsouk, Mike R Garde, Ryan J Liu, Baohong Nixon, Benjamin R Manalo, Annabelle E Becker, Jason R Crawley, Scott W Bader, David M Tyska, Matthew J Liu, Qi Gutzman, Jennifer H Burnette, Dylan T Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes |
title | Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes |
title_full | Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes |
title_fullStr | Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes |
title_full_unstemmed | Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes |
title_short | Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes |
title_sort | muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307863/ https://www.ncbi.nlm.nih.gov/pubmed/30540249 http://dx.doi.org/10.7554/eLife.42144 |
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