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A broadly applicable COI primer pair and an efficient single‐tube amplicon library preparation protocol for metabarcoding

The nucleotide variation in the cytochrome c oxidase subunit I (COI) gene makes it ideal for assigning sequences to species. However, this variability also makes it difficult to design truly universal primers. Here, we present the forward primer “Sauron‐S878,” specifically designed to facilitate lib...

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Detalles Bibliográficos
Autores principales: Rennstam Rubbmark, Oskar, Sint, Daniela, Horngacher, Nina, Traugott, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308894/
https://www.ncbi.nlm.nih.gov/pubmed/30619549
http://dx.doi.org/10.1002/ece3.4520