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Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads

Eight yeast strains of the genera Saccharomyces and Kluyveromyces were screened to ferment high lactose-load cheese whey permeate (CWP) (>130 g/L lactose) without nutrient supplementation. The fermentation conditions (temperature, pH and time) were optimized to maximize the fermentation performan...

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Autores principales: Díez-Antolínez, Rebeca, Hijosa-Valsero, María, Paniagua-García, Ana I., Garita-Cambronero, Jerson, Gómez, Xiomar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312371/
https://www.ncbi.nlm.nih.gov/pubmed/30596755
http://dx.doi.org/10.1371/journal.pone.0210002
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author Díez-Antolínez, Rebeca
Hijosa-Valsero, María
Paniagua-García, Ana I.
Garita-Cambronero, Jerson
Gómez, Xiomar
author_facet Díez-Antolínez, Rebeca
Hijosa-Valsero, María
Paniagua-García, Ana I.
Garita-Cambronero, Jerson
Gómez, Xiomar
author_sort Díez-Antolínez, Rebeca
collection PubMed
description Eight yeast strains of the genera Saccharomyces and Kluyveromyces were screened to ferment high lactose-load cheese whey permeate (CWP) (>130 g/L lactose) without nutrient supplementation. The fermentation conditions (temperature, pH and time) were optimized to maximize the fermentation performance (ethanol titer, ethanol yield and lactose consumption) for the two preselected strains, K. marxianus DSM 5422 and S. cerevisiae Ethanol Red, using a response surface methodology (RSM). Under optimized conditions, K. marxianus DSM 5422 attained ethanol titers of 6% (v/v) in only 44 h. Moreover, the feasibility of immobilizing this strain on four different inorganic supports (plastic, glass and Tygon silicone Raschig rings and alumina beads) was assessed. Glass Raschig rings and alumina beads showed a more stable performance over time, yielding ethanol titers of 60 g/L during 1,000 hours, which remarkably reduces yeast cultivation costs. Results demonstrate the feasibility of using CWP for successful ethanol production in a simple and economical process, which represents an attractive alternative for waste treatment in dairy industries.
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spelling pubmed-63123712019-01-08 Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads Díez-Antolínez, Rebeca Hijosa-Valsero, María Paniagua-García, Ana I. Garita-Cambronero, Jerson Gómez, Xiomar PLoS One Research Article Eight yeast strains of the genera Saccharomyces and Kluyveromyces were screened to ferment high lactose-load cheese whey permeate (CWP) (>130 g/L lactose) without nutrient supplementation. The fermentation conditions (temperature, pH and time) were optimized to maximize the fermentation performance (ethanol titer, ethanol yield and lactose consumption) for the two preselected strains, K. marxianus DSM 5422 and S. cerevisiae Ethanol Red, using a response surface methodology (RSM). Under optimized conditions, K. marxianus DSM 5422 attained ethanol titers of 6% (v/v) in only 44 h. Moreover, the feasibility of immobilizing this strain on four different inorganic supports (plastic, glass and Tygon silicone Raschig rings and alumina beads) was assessed. Glass Raschig rings and alumina beads showed a more stable performance over time, yielding ethanol titers of 60 g/L during 1,000 hours, which remarkably reduces yeast cultivation costs. Results demonstrate the feasibility of using CWP for successful ethanol production in a simple and economical process, which represents an attractive alternative for waste treatment in dairy industries. Public Library of Science 2018-12-31 /pmc/articles/PMC6312371/ /pubmed/30596755 http://dx.doi.org/10.1371/journal.pone.0210002 Text en © 2018 Díez-Antolínez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Díez-Antolínez, Rebeca
Hijosa-Valsero, María
Paniagua-García, Ana I.
Garita-Cambronero, Jerson
Gómez, Xiomar
Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads
title Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads
title_full Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads
title_fullStr Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads
title_full_unstemmed Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads
title_short Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads
title_sort yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312371/
https://www.ncbi.nlm.nih.gov/pubmed/30596755
http://dx.doi.org/10.1371/journal.pone.0210002
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