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Rapid and user-friendly open-source CRISPR/Cas9 system for single- or multi-site editing of tomato genome
CRISPR/Cas9-induced genome editing is a powerful tool for studying gene function in a variety of organisms, including plants. Using multi-sgRNAs to target one or more genes is helpful to improve the efficacy of gene editing and facilitate multi-gene editing. Here, we describe a CRISPR/Cas9 system wh...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312546/ https://www.ncbi.nlm.nih.gov/pubmed/30603093 http://dx.doi.org/10.1038/s41438-018-0082-6 |