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Rapid and user-friendly open-source CRISPR/Cas9 system for single- or multi-site editing of tomato genome

CRISPR/Cas9-induced genome editing is a powerful tool for studying gene function in a variety of organisms, including plants. Using multi-sgRNAs to target one or more genes is helpful to improve the efficacy of gene editing and facilitate multi-gene editing. Here, we describe a CRISPR/Cas9 system wh...

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Detalles Bibliográficos
Autores principales: Hu, Nan, Xian, Zhiqiang, Li, Ning, Liu, Yudong, Huang, Wei, Yan, Fang, Su, Deding, Chen, Jingxuan, Li, Zhengguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312546/
https://www.ncbi.nlm.nih.gov/pubmed/30603093
http://dx.doi.org/10.1038/s41438-018-0082-6