A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2α

Many studies require the detection and relative quantitation of proteins from cell culture samples using immunoblotting. Limiting factors are the cost of protease inhibitors, the time required to break cells and generate samples, as well as the high risk of protein loss during cell breakage procedur...

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Detalles Bibliográficos
Autores principales: Silva, Richard C., Castilho, Beatriz A., Sattlegger, Evelyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314271/
https://www.ncbi.nlm.nih.gov/pubmed/30619721
http://dx.doi.org/10.1016/j.mex.2017.10.008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314271/
https://www.ncbi.nlm.nih.gov/pubmed/30619721
http://dx.doi.org/10.1016/j.mex.2017.10.008

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