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Tools and protocol for quantification of myosin phosphorylation with MRM-MS

The phosphorylation of myosin regulatory light chain (LC20) at Thr18 and Ser19 is positively correlated with tension development in smooth muscle tissue, and the molar stoichiometry of LC20 phosphorylation is commonly profiled as a measure of smooth muscle contractility. We provide details for a new...

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Detalles Bibliográficos
Autores principales: MacDonald, Justin A., Ulke-Lemée, Annegret, Chappellaz, Mona, Segboer, Hayden
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314968/
https://www.ncbi.nlm.nih.gov/pubmed/30622916
http://dx.doi.org/10.1016/j.mex.2018.05.004