Tools and protocol for quantification of myosin phosphorylation with MRM-MS

The phosphorylation of myosin regulatory light chain (LC20) at Thr18 and Ser19 is positively correlated with tension development in smooth muscle tissue, and the molar stoichiometry of LC20 phosphorylation is commonly profiled as a measure of smooth muscle contractility. We provide details for a new...

Descripción completa

Detalles Bibliográficos
Autores principales: MacDonald, Justin A., Ulke-Lemée, Annegret, Chappellaz, Mona, Segboer, Hayden
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314968/
https://www.ncbi.nlm.nih.gov/pubmed/30622916
http://dx.doi.org/10.1016/j.mex.2018.05.004
Descripción
Sumario:The phosphorylation of myosin regulatory light chain (LC20) at Thr18 and Ser19 is positively correlated with tension development in smooth muscle tissue, and the molar stoichiometry of LC20 phosphorylation is commonly profiled as a measure of smooth muscle contractility. We provide details for a newly applied multiple reaction monitoring (MRM)-mass spectrometry (MS) method for the quantification of LC20 phosphorylation at Thr18 and Ser19. This MRM-MS method provides a robust alternative to antibody-based detection systems (such as Phos-Tag SDS-PAGE) for the quantification of LC20 phosphorylation.

Ejemplares similares