Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening

We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green and red FRET pairs (GFP/RFP), FPs that emit at longe...

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Detalles Bibliográficos
Autores principales: Schaaf, Tory M., Li, Ang, Grant, Benjamin D., Peterson, Kurt, Yuen, Samantha, Bawaskar, Prachi, Kleinboehl, Evan, Li, Ji, Thomas, David D., Gillispie, Gregory D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315989/
https://www.ncbi.nlm.nih.gov/pubmed/30352972
http://dx.doi.org/10.3390/bios8040099

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315989/
https://www.ncbi.nlm.nih.gov/pubmed/30352972
http://dx.doi.org/10.3390/bios8040099

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