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Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening
We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green and red FRET pairs (GFP/RFP), FPs that emit at longe...
Autores principales: | Schaaf, Tory M., Li, Ang, Grant, Benjamin D., Peterson, Kurt, Yuen, Samantha, Bawaskar, Prachi, Kleinboehl, Evan, Li, Ji, Thomas, David D., Gillispie, Gregory D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315989/ https://www.ncbi.nlm.nih.gov/pubmed/30352972 http://dx.doi.org/10.3390/bios8040099 |
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