Cargando…

Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning

Lactic acid bacteria (LAB) is one of the main classes of acid-producing organisms in the food industry, and they play a vital part in many food and feed fermentations. We isolated and performed molecular identification of LAB from raw camel's milk and assessed their protective effects against p...

Descripción completa

Detalles Bibliográficos
Autores principales: Edalati, E., Saneei, B., Alizadeh, M., Hosseini, S.S., Zahedi Bialvaei, A., Taheri, K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317326/
https://www.ncbi.nlm.nih.gov/pubmed/30622712
http://dx.doi.org/10.1016/j.nmni.2018.11.008
_version_ 1783384733379461120
author Edalati, E.
Saneei, B.
Alizadeh, M.
Hosseini, S.S.
Zahedi Bialvaei, A.
Taheri, K.
author_facet Edalati, E.
Saneei, B.
Alizadeh, M.
Hosseini, S.S.
Zahedi Bialvaei, A.
Taheri, K.
author_sort Edalati, E.
collection PubMed
description Lactic acid bacteria (LAB) is one of the main classes of acid-producing organisms in the food industry, and they play a vital part in many food and feed fermentations. We isolated and performed molecular identification of LAB from raw camel's milk and assessed their protective effects against pathogenicity induced by Staphylococcus aureus and Escherichia coli. Fourteen samples of camel's milk were obtained from several districts under aseptic conditions. Bacteria isolation was performed by plating the samples on selective media. Isolates were identified by amplification of the 16S ribosomal RNA by PCR and sequencing. A total of 32 isolates were randomly picked, eight of which were analysed in this study. On the basis of phenotypic and genotypic methods, isolated LAB was included Leuconostoc mesenteroides, Lactobacillus plantarum, Weissella paramesenteroides and Weissella confuse. Antagonistic activity of isolated LAB against two pathogenic bacteria showed that they had more inhibitory activity against S. aureus subsp. aureus PTCC 1431 than E. coli ATCC 25922. This study discovered that raw camel's milk obtained from three districts of Kerman province contain LAB bacteria that have antagonistic properties on S. aureus.
format Online
Article
Text
id pubmed-6317326
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-63173262019-01-08 Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning Edalati, E. Saneei, B. Alizadeh, M. Hosseini, S.S. Zahedi Bialvaei, A. Taheri, K. New Microbes New Infect Original Article Lactic acid bacteria (LAB) is one of the main classes of acid-producing organisms in the food industry, and they play a vital part in many food and feed fermentations. We isolated and performed molecular identification of LAB from raw camel's milk and assessed their protective effects against pathogenicity induced by Staphylococcus aureus and Escherichia coli. Fourteen samples of camel's milk were obtained from several districts under aseptic conditions. Bacteria isolation was performed by plating the samples on selective media. Isolates were identified by amplification of the 16S ribosomal RNA by PCR and sequencing. A total of 32 isolates were randomly picked, eight of which were analysed in this study. On the basis of phenotypic and genotypic methods, isolated LAB was included Leuconostoc mesenteroides, Lactobacillus plantarum, Weissella paramesenteroides and Weissella confuse. Antagonistic activity of isolated LAB against two pathogenic bacteria showed that they had more inhibitory activity against S. aureus subsp. aureus PTCC 1431 than E. coli ATCC 25922. This study discovered that raw camel's milk obtained from three districts of Kerman province contain LAB bacteria that have antagonistic properties on S. aureus. Elsevier 2018-12-06 /pmc/articles/PMC6317326/ /pubmed/30622712 http://dx.doi.org/10.1016/j.nmni.2018.11.008 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Edalati, E.
Saneei, B.
Alizadeh, M.
Hosseini, S.S.
Zahedi Bialvaei, A.
Taheri, K.
Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning
title Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning
title_full Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning
title_fullStr Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning
title_full_unstemmed Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning
title_short Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning
title_sort isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317326/
https://www.ncbi.nlm.nih.gov/pubmed/30622712
http://dx.doi.org/10.1016/j.nmni.2018.11.008
work_keys_str_mv AT edalatie isolationofprobioticbacteriafromrawcamelsmilkandtheirantagonisticeffectsontwobacteriacausingfoodpoisoning
AT saneeib isolationofprobioticbacteriafromrawcamelsmilkandtheirantagonisticeffectsontwobacteriacausingfoodpoisoning
AT alizadehm isolationofprobioticbacteriafromrawcamelsmilkandtheirantagonisticeffectsontwobacteriacausingfoodpoisoning
AT hosseiniss isolationofprobioticbacteriafromrawcamelsmilkandtheirantagonisticeffectsontwobacteriacausingfoodpoisoning
AT zahedibialvaeia isolationofprobioticbacteriafromrawcamelsmilkandtheirantagonisticeffectsontwobacteriacausingfoodpoisoning
AT taherik isolationofprobioticbacteriafromrawcamelsmilkandtheirantagonisticeffectsontwobacteriacausingfoodpoisoning