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Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway

AIM: To investigate the effects of catalpol on muscular atrophy induced by sciatic nerve crush injury (SNCI). METHODS: Seventy male Kunming mice were randomized into five groups (n=10): model, sham, catalpol (Cat), rapamycin (Rapa), and catalpol+rapamycin (Rapa+Cat). The ratio of gastrocnemius muscl...

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Autores principales: Wang, Yuan, Shao, Yali, Gao, Yuqing, Wan, Guoran, Wan, Dong, Zhu, Huifeng, Qiu, Yan, Ye, Xiyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319426/
https://www.ncbi.nlm.nih.gov/pubmed/30643390
http://dx.doi.org/10.2147/DDDT.S188968
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author Wang, Yuan
Shao, Yali
Gao, Yuqing
Wan, Guoran
Wan, Dong
Zhu, Huifeng
Qiu, Yan
Ye, Xiyue
author_facet Wang, Yuan
Shao, Yali
Gao, Yuqing
Wan, Guoran
Wan, Dong
Zhu, Huifeng
Qiu, Yan
Ye, Xiyue
author_sort Wang, Yuan
collection PubMed
description AIM: To investigate the effects of catalpol on muscular atrophy induced by sciatic nerve crush injury (SNCI). METHODS: Seventy male Kunming mice were randomized into five groups (n=10): model, sham, catalpol (Cat), rapamycin (Rapa), and catalpol+rapamycin (Rapa+Cat). The ratio of gastrocnemius muscle wet weight (right/left, R/L) between the operated leg (right) and the normal leg (left) was calculated, and acetylcholinesterase (AChE) immunohistochemistry assays were performed to observe the change of motor end plate (MEP), along with the sizes of denervated and innervated muscle fibers. The expression levels of LC3II, TUNEL, BAX/BCL-2, LC3II/LC3I and P62, Beclin1, mTOR, and p-mTOR (ser2448) proteins in muscle were examined by fluorescence immunohistochemistry or Western blotting. RESULTS: Results show that catalpol improved the results of the grid walking tests by reducing the percentage of foot slips, which increased the gastrocnemius muscle wet weight (R/L), enhanced AChE expression at the MEP, and enlarged the section area of the muscle. The expression of LC3II and TUNEL was significantly inhibited by catalpol. The BAX/BCL-2 ratio was significantly increased in muscles of denervated and control groups. Lower LC3II/LC3I and BAX/BCL-2 ratios in denervated muscles were also detected after catalpol treatment. CONCLUSION: These results indicated that apoptosis and autophagy play a role in the regulation of denervation-induced muscle atrophy after SNCI, and catalpol alleviates muscle atrophy through the regulation of muscle apoptosis and autophagy via the mTOR signaling pathway.
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spelling pubmed-63194262019-01-14 Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway Wang, Yuan Shao, Yali Gao, Yuqing Wan, Guoran Wan, Dong Zhu, Huifeng Qiu, Yan Ye, Xiyue Drug Des Devel Ther Original Research AIM: To investigate the effects of catalpol on muscular atrophy induced by sciatic nerve crush injury (SNCI). METHODS: Seventy male Kunming mice were randomized into five groups (n=10): model, sham, catalpol (Cat), rapamycin (Rapa), and catalpol+rapamycin (Rapa+Cat). The ratio of gastrocnemius muscle wet weight (right/left, R/L) between the operated leg (right) and the normal leg (left) was calculated, and acetylcholinesterase (AChE) immunohistochemistry assays were performed to observe the change of motor end plate (MEP), along with the sizes of denervated and innervated muscle fibers. The expression levels of LC3II, TUNEL, BAX/BCL-2, LC3II/LC3I and P62, Beclin1, mTOR, and p-mTOR (ser2448) proteins in muscle were examined by fluorescence immunohistochemistry or Western blotting. RESULTS: Results show that catalpol improved the results of the grid walking tests by reducing the percentage of foot slips, which increased the gastrocnemius muscle wet weight (R/L), enhanced AChE expression at the MEP, and enlarged the section area of the muscle. The expression of LC3II and TUNEL was significantly inhibited by catalpol. The BAX/BCL-2 ratio was significantly increased in muscles of denervated and control groups. Lower LC3II/LC3I and BAX/BCL-2 ratios in denervated muscles were also detected after catalpol treatment. CONCLUSION: These results indicated that apoptosis and autophagy play a role in the regulation of denervation-induced muscle atrophy after SNCI, and catalpol alleviates muscle atrophy through the regulation of muscle apoptosis and autophagy via the mTOR signaling pathway. Dove Medical Press 2018-12-31 /pmc/articles/PMC6319426/ /pubmed/30643390 http://dx.doi.org/10.2147/DDDT.S188968 Text en © 2019 Wang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Wang, Yuan
Shao, Yali
Gao, Yuqing
Wan, Guoran
Wan, Dong
Zhu, Huifeng
Qiu, Yan
Ye, Xiyue
Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway
title Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway
title_full Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway
title_fullStr Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway
title_full_unstemmed Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway
title_short Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway
title_sort catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces bax/bcl2 ratio via mtor pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319426/
https://www.ncbi.nlm.nih.gov/pubmed/30643390
http://dx.doi.org/10.2147/DDDT.S188968
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