Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. In this work, we compared their activities by targeting maize glossy2 gene coding region that has overlapping sequences recognized by both nucleases. We introduced constructs carrying SpCas9‐guide RN...

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Detalles Bibliográficos
Autores principales: Lee, Keunsub, Zhang, Yingxiao, Kleinstiver, Benjamin P., Guo, Jimmy A., Aryee, Martin J., Miller, Jonah, Malzahn, Aimee, Zarecor, Scott, Lawrence‐Dill, Carolyn J., Joung, J. Keith, Qi, Yiping, Wang, Kan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320322/
https://www.ncbi.nlm.nih.gov/pubmed/29972722
http://dx.doi.org/10.1111/pbi.12982

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320322/
https://www.ncbi.nlm.nih.gov/pubmed/29972722
http://dx.doi.org/10.1111/pbi.12982

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