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Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box
Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluores...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321608/ https://www.ncbi.nlm.nih.gov/pubmed/30518054 http://dx.doi.org/10.3390/ijms19123865 |
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author | Moosa, Mahdi Muhammad Tsoi, Phoebe S. Choi, Kyoung-Jae Ferreon, Allan Chris M. Ferreon, Josephine C. |
author_facet | Moosa, Mahdi Muhammad Tsoi, Phoebe S. Choi, Kyoung-Jae Ferreon, Allan Chris M. Ferreon, Josephine C. |
author_sort | Moosa, Mahdi Muhammad |
collection | PubMed |
description | Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluorescence spectroscopy, we show that Sox2 alone can modulate DNA structural landscape in a dosage-dependent manner. We propose that such stoichiometric tuning of regulatory DNAs is crucial to the diverse biological functions of Sox2, and represents a generic mechanism of conferring functional plasticity and multiplicity to transcription factors. |
format | Online Article Text |
id | pubmed-6321608 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63216082019-01-07 Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box Moosa, Mahdi Muhammad Tsoi, Phoebe S. Choi, Kyoung-Jae Ferreon, Allan Chris M. Ferreon, Josephine C. Int J Mol Sci Communication Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluorescence spectroscopy, we show that Sox2 alone can modulate DNA structural landscape in a dosage-dependent manner. We propose that such stoichiometric tuning of regulatory DNAs is crucial to the diverse biological functions of Sox2, and represents a generic mechanism of conferring functional plasticity and multiplicity to transcription factors. MDPI 2018-12-04 /pmc/articles/PMC6321608/ /pubmed/30518054 http://dx.doi.org/10.3390/ijms19123865 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Moosa, Mahdi Muhammad Tsoi, Phoebe S. Choi, Kyoung-Jae Ferreon, Allan Chris M. Ferreon, Josephine C. Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box |
title | Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box |
title_full | Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box |
title_fullStr | Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box |
title_full_unstemmed | Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box |
title_short | Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box |
title_sort | direct single-molecule observation of sequential dna bending transitions by the sox2 hmg box |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321608/ https://www.ncbi.nlm.nih.gov/pubmed/30518054 http://dx.doi.org/10.3390/ijms19123865 |
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