Cargando…

Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box

Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluores...

Descripción completa

Detalles Bibliográficos
Autores principales: Moosa, Mahdi Muhammad, Tsoi, Phoebe S., Choi, Kyoung-Jae, Ferreon, Allan Chris M., Ferreon, Josephine C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321608/
https://www.ncbi.nlm.nih.gov/pubmed/30518054
http://dx.doi.org/10.3390/ijms19123865
_version_ 1783385482963451904
author Moosa, Mahdi Muhammad
Tsoi, Phoebe S.
Choi, Kyoung-Jae
Ferreon, Allan Chris M.
Ferreon, Josephine C.
author_facet Moosa, Mahdi Muhammad
Tsoi, Phoebe S.
Choi, Kyoung-Jae
Ferreon, Allan Chris M.
Ferreon, Josephine C.
author_sort Moosa, Mahdi Muhammad
collection PubMed
description Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluorescence spectroscopy, we show that Sox2 alone can modulate DNA structural landscape in a dosage-dependent manner. We propose that such stoichiometric tuning of regulatory DNAs is crucial to the diverse biological functions of Sox2, and represents a generic mechanism of conferring functional plasticity and multiplicity to transcription factors.
format Online
Article
Text
id pubmed-6321608
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63216082019-01-07 Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box Moosa, Mahdi Muhammad Tsoi, Phoebe S. Choi, Kyoung-Jae Ferreon, Allan Chris M. Ferreon, Josephine C. Int J Mol Sci Communication Sox2 is a pioneer transcription factor that initiates cell fate reprogramming through locus-specific differential regulation. Mechanistically, it was assumed that Sox2 achieves its regulatory diversity via heterodimerization with partner transcription factors. Here, utilizing single-molecule fluorescence spectroscopy, we show that Sox2 alone can modulate DNA structural landscape in a dosage-dependent manner. We propose that such stoichiometric tuning of regulatory DNAs is crucial to the diverse biological functions of Sox2, and represents a generic mechanism of conferring functional plasticity and multiplicity to transcription factors. MDPI 2018-12-04 /pmc/articles/PMC6321608/ /pubmed/30518054 http://dx.doi.org/10.3390/ijms19123865 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Moosa, Mahdi Muhammad
Tsoi, Phoebe S.
Choi, Kyoung-Jae
Ferreon, Allan Chris M.
Ferreon, Josephine C.
Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box
title Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box
title_full Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box
title_fullStr Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box
title_full_unstemmed Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box
title_short Direct Single-Molecule Observation of Sequential DNA Bending Transitions by the Sox2 HMG Box
title_sort direct single-molecule observation of sequential dna bending transitions by the sox2 hmg box
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321608/
https://www.ncbi.nlm.nih.gov/pubmed/30518054
http://dx.doi.org/10.3390/ijms19123865
work_keys_str_mv AT moosamahdimuhammad directsinglemoleculeobservationofsequentialdnabendingtransitionsbythesox2hmgbox
AT tsoiphoebes directsinglemoleculeobservationofsequentialdnabendingtransitionsbythesox2hmgbox
AT choikyoungjae directsinglemoleculeobservationofsequentialdnabendingtransitionsbythesox2hmgbox
AT ferreonallanchrism directsinglemoleculeobservationofsequentialdnabendingtransitionsbythesox2hmgbox
AT ferreonjosephinec directsinglemoleculeobservationofsequentialdnabendingtransitionsbythesox2hmgbox