Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs

Molecular tools to target RNA site-specifically allow recoding of RNA information and processing. SNAP-tagged deaminases, guided by a chemically stabilized guideRNA, enable the simultaneous editing of targeted adenosine to inosine in several endogenous transcripts, with high efficiency (up to 90%),...

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Detalles Bibliográficos
Autores principales: Vogel, Paul, Moschref, Matin, Li, Qin, Merkle, Tobias, Selvasaravanan, Karthika D., Li, Jin Billy, Stafforst, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322650/
https://www.ncbi.nlm.nih.gov/pubmed/29967493
http://dx.doi.org/10.1038/s41592-018-0017-z
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author Vogel, Paul
Moschref, Matin
Li, Qin
Merkle, Tobias
Selvasaravanan, Karthika D.
Li, Jin Billy
Stafforst, Thorsten
author_facet Vogel, Paul
Moschref, Matin
Li, Qin
Merkle, Tobias
Selvasaravanan, Karthika D.
Li, Jin Billy
Stafforst, Thorsten
author_sort Vogel, Paul
collection PubMed
description Molecular tools to target RNA site-specifically allow recoding of RNA information and processing. SNAP-tagged deaminases, guided by a chemically stabilized guideRNA, enable the simultaneous editing of targeted adenosine to inosine in several endogenous transcripts, with high efficiency (up to 90%), high potency, sufficient duration, and high precision. We applied SNAP-ADARs for the efficient and concurrent editing of two disease-relevant signaling transcripts, KRAS and STAT1. We also show improved performance compared to the recently described Cas13b-ADAR.
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spelling pubmed-63226502019-01-07 Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs Vogel, Paul Moschref, Matin Li, Qin Merkle, Tobias Selvasaravanan, Karthika D. Li, Jin Billy Stafforst, Thorsten Nat Methods Article Molecular tools to target RNA site-specifically allow recoding of RNA information and processing. SNAP-tagged deaminases, guided by a chemically stabilized guideRNA, enable the simultaneous editing of targeted adenosine to inosine in several endogenous transcripts, with high efficiency (up to 90%), high potency, sufficient duration, and high precision. We applied SNAP-ADARs for the efficient and concurrent editing of two disease-relevant signaling transcripts, KRAS and STAT1. We also show improved performance compared to the recently described Cas13b-ADAR. 2018-07-02 2018-07 /pmc/articles/PMC6322650/ /pubmed/29967493 http://dx.doi.org/10.1038/s41592-018-0017-z Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Vogel, Paul
Moschref, Matin
Li, Qin
Merkle, Tobias
Selvasaravanan, Karthika D.
Li, Jin Billy
Stafforst, Thorsten
Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs
title Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs
title_full Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs
title_fullStr Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs
title_full_unstemmed Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs
title_short Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs
title_sort efficient and precise editing of endogenous transcripts with snap-tagged adars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322650/
https://www.ncbi.nlm.nih.gov/pubmed/29967493
http://dx.doi.org/10.1038/s41592-018-0017-z
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