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CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis
CRISPR-Cas systems have become widely used across all fields of biology as a genome engineering tool. With its recent demonstration in the Gram positive industrial workhorse Bacillus subtilis, this tool has become an attractive option for rapid, markerless strain engineering of industrial production...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322745/ https://www.ncbi.nlm.nih.gov/pubmed/30615645 http://dx.doi.org/10.1371/journal.pone.0210121 |