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Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions

Production of recombinant proteins such as antibody fragments in the periplasm of the bacterium Escherichia coli has a number of advantages, including the ability to form disulphide bonds, aiding correct folding, and the relative ease of release and subsequent capture and purification. In this study...

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Detalles Bibliográficos
Autores principales: Kasli, Ikhlaas M., Thomas, Owen R. T., Overton, Tim W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323050/
https://www.ncbi.nlm.nih.gov/pubmed/30617435
http://dx.doi.org/10.1186/s13568-018-0727-8