Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex

BACKGROUND: CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome. RESULT...

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Detalles Bibliográficos
Autores principales: Hao, Zhenzhen, Su, Xiaoyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325762/
https://www.ncbi.nlm.nih.gov/pubmed/30626373
http://dx.doi.org/10.1186/s12896-018-0498-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325762/
https://www.ncbi.nlm.nih.gov/pubmed/30626373
http://dx.doi.org/10.1186/s12896-018-0498-y

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