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Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex
BACKGROUND: CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome. RESULT...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325762/ https://www.ncbi.nlm.nih.gov/pubmed/30626373 http://dx.doi.org/10.1186/s12896-018-0498-y |