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Efficient Homologous Recombination in Mice Using Long Single Stranded DNA and CRISPR Cas9 Nickase
The CRISPR/Cas9 nickase mutant is less prone to off-target double-strand (ds)DNA breaks than wild-type Cas9 because to produce dsDNA cleavage it requires two guide RNAs to target the nickase to nearby opposing strands. Like wild-type Cas9 lesions, these staggered lesions are repaired by either non-h...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Genetics Society of America
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325892/ https://www.ncbi.nlm.nih.gov/pubmed/30504134 http://dx.doi.org/10.1534/g3.118.200758 |