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Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

Bacterial CRISPR systems have been widely adopted to create operator-specified site-specific nucleases. Such nuclease action commonly results in loss-of-function alleles, facilitating functional analysis of genes and gene families We conducted a systematic comparison of components and T-DNA architec...

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Detalles Bibliográficos
Autores principales: Castel, Baptiste, Tomlinson, Laurence, Locci, Federica, Yang, Ying, Jones, Jonathan D. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326418/
https://www.ncbi.nlm.nih.gov/pubmed/30625150
http://dx.doi.org/10.1371/journal.pone.0204778