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Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis
Bacterial CRISPR systems have been widely adopted to create operator-specified site-specific nucleases. Such nuclease action commonly results in loss-of-function alleles, facilitating functional analysis of genes and gene families We conducted a systematic comparison of components and T-DNA architec...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326418/ https://www.ncbi.nlm.nih.gov/pubmed/30625150 http://dx.doi.org/10.1371/journal.pone.0204778 |