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Structure and function of an ectopic Polycomb chromatin domain
Polycomb group proteins (PcGs) drive target gene repression and form large chromatin domains. In Drosophila, DNA elements known as Polycomb group response elements (PREs) recruit PcGs to the DNA. We have shown that, within the invected-engrailed (inv-en) Polycomb domain, strong, constitutive PREs ar...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326746/ https://www.ncbi.nlm.nih.gov/pubmed/30662949 http://dx.doi.org/10.1126/sciadv.aau9739 |
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author | De, Sandip Cheng, Yuzhong Sun, Ming-an Gehred, Natalie D. Kassis, Judith A. |
author_facet | De, Sandip Cheng, Yuzhong Sun, Ming-an Gehred, Natalie D. Kassis, Judith A. |
author_sort | De, Sandip |
collection | PubMed |
description | Polycomb group proteins (PcGs) drive target gene repression and form large chromatin domains. In Drosophila, DNA elements known as Polycomb group response elements (PREs) recruit PcGs to the DNA. We have shown that, within the invected-engrailed (inv-en) Polycomb domain, strong, constitutive PREs are dispensable for Polycomb domain structure and function. We suggest that the endogenous chromosomal location imparts stability to this Polycomb domain. To test this possibility, a 79-kb en transgene was inserted into other chromosomal locations. This transgene is functional and forms a Polycomb domain. The spreading of the H3K27me3 repressive mark, characteristic of PcG domains, varies depending on the chromatin context of the transgene. Unlike at the endogenous locus, deletion of the strong, constitutive PREs from the transgene leads to both loss- and gain-of function phenotypes, demonstrating the important role of these regulatory elements. Our data show that chromatin context plays an important role in Polycomb domain structure and function. |
format | Online Article Text |
id | pubmed-6326746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63267462019-01-18 Structure and function of an ectopic Polycomb chromatin domain De, Sandip Cheng, Yuzhong Sun, Ming-an Gehred, Natalie D. Kassis, Judith A. Sci Adv Research Articles Polycomb group proteins (PcGs) drive target gene repression and form large chromatin domains. In Drosophila, DNA elements known as Polycomb group response elements (PREs) recruit PcGs to the DNA. We have shown that, within the invected-engrailed (inv-en) Polycomb domain, strong, constitutive PREs are dispensable for Polycomb domain structure and function. We suggest that the endogenous chromosomal location imparts stability to this Polycomb domain. To test this possibility, a 79-kb en transgene was inserted into other chromosomal locations. This transgene is functional and forms a Polycomb domain. The spreading of the H3K27me3 repressive mark, characteristic of PcG domains, varies depending on the chromatin context of the transgene. Unlike at the endogenous locus, deletion of the strong, constitutive PREs from the transgene leads to both loss- and gain-of function phenotypes, demonstrating the important role of these regulatory elements. Our data show that chromatin context plays an important role in Polycomb domain structure and function. American Association for the Advancement of Science 2019-01-09 /pmc/articles/PMC6326746/ /pubmed/30662949 http://dx.doi.org/10.1126/sciadv.aau9739 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles De, Sandip Cheng, Yuzhong Sun, Ming-an Gehred, Natalie D. Kassis, Judith A. Structure and function of an ectopic Polycomb chromatin domain |
title | Structure and function of an ectopic Polycomb chromatin domain |
title_full | Structure and function of an ectopic Polycomb chromatin domain |
title_fullStr | Structure and function of an ectopic Polycomb chromatin domain |
title_full_unstemmed | Structure and function of an ectopic Polycomb chromatin domain |
title_short | Structure and function of an ectopic Polycomb chromatin domain |
title_sort | structure and function of an ectopic polycomb chromatin domain |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326746/ https://www.ncbi.nlm.nih.gov/pubmed/30662949 http://dx.doi.org/10.1126/sciadv.aau9739 |
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