Targeting individual cells by barcode in pooled sequence libraries

Transcriptional profiling of thousands of single cells in parallel by RNA-seq is now routine. However, due to reliance on pooled library preparation, targeting analysis to particular cells of interest is difficult. Here, we present a multiplexed PCR method for targeted sequencing of select cells fro...

Descripción completa

Detalles Bibliográficos
Autores principales: Ranu, Navpreet, Villani, Alexandra-Chloé, Hacohen, Nir, Blainey, Paul C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326790/
https://www.ncbi.nlm.nih.gov/pubmed/30256981
http://dx.doi.org/10.1093/nar/gky856

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326790/
https://www.ncbi.nlm.nih.gov/pubmed/30256981
http://dx.doi.org/10.1093/nar/gky856

Ejemplares similares