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Structure, subunit organization and behavior of the asymmetric Type IIT restriction endonuclease BbvCI
BbvCI, a Type IIT restriction endonuclease, recognizes and cleaves the seven base pair sequence 5′-CCTCAGC-3′, generating 3-base, 5′-overhangs. BbvCI is composed of two protein subunits, each containing one catalytic site. Either site can be inactivated by mutation resulting in enzyme variants that...
Autores principales: | Shen, Betty W, Doyle, Lindsey, Bradley, Phil, Heiter, Daniel F, Lunnen, Keith D, Wilson, Geoffrey G, Stoddard, Barry L |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326814/ https://www.ncbi.nlm.nih.gov/pubmed/30395313 http://dx.doi.org/10.1093/nar/gky1059 |
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