Cargando…
Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene
Discovery and characterization of broadly neutralizing antibodies (bnAbs) to the influenza hemagglutinin (HA) stem have provided insights for the development of a universal flu vaccine. Identification of signature features common to bnAbs from different individuals will be key to guiding immunogen d...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327842/ https://www.ncbi.nlm.nih.gov/pubmed/30308159 http://dx.doi.org/10.1016/j.chom.2018.09.010 |
_version_ | 1783386551892312064 |
---|---|
author | Wu, Nicholas C. Yamayoshi, Seiya Ito, Mutsumi Uraki, Ryuta Kawaoka, Yoshihiro Wilson, Ian A. |
author_facet | Wu, Nicholas C. Yamayoshi, Seiya Ito, Mutsumi Uraki, Ryuta Kawaoka, Yoshihiro Wilson, Ian A. |
author_sort | Wu, Nicholas C. |
collection | PubMed |
description | Discovery and characterization of broadly neutralizing antibodies (bnAbs) to the influenza hemagglutinin (HA) stem have provided insights for the development of a universal flu vaccine. Identification of signature features common to bnAbs from different individuals will be key to guiding immunogen design. S9-3-37 is a bnAb isolated from a healthy H5N1 vaccinee. Here, structural characterization reveals that the D3-9 gene segment of S9-3-37 contributes most of the interaction surface with the highly conserved stem epitope on HA. Comparison with other influenza bnAb crystal structures indicates that the D3-9 segment provides a general mechanism for targeting HA stem. Interestingly, such bnAbs can approach the HA stem with vastly different angles and orientations. Moreover, D3-9 can be translated in different reading frames in different bnAbs yet still target the same HA stem pocket. Thus, the D3-9 gene segment in the human immune repertoire can provide a robust defense against influenza virus. |
format | Online Article Text |
id | pubmed-6327842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63278422019-10-10 Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene Wu, Nicholas C. Yamayoshi, Seiya Ito, Mutsumi Uraki, Ryuta Kawaoka, Yoshihiro Wilson, Ian A. Cell Host Microbe Article Discovery and characterization of broadly neutralizing antibodies (bnAbs) to the influenza hemagglutinin (HA) stem have provided insights for the development of a universal flu vaccine. Identification of signature features common to bnAbs from different individuals will be key to guiding immunogen design. S9-3-37 is a bnAb isolated from a healthy H5N1 vaccinee. Here, structural characterization reveals that the D3-9 gene segment of S9-3-37 contributes most of the interaction surface with the highly conserved stem epitope on HA. Comparison with other influenza bnAb crystal structures indicates that the D3-9 segment provides a general mechanism for targeting HA stem. Interestingly, such bnAbs can approach the HA stem with vastly different angles and orientations. Moreover, D3-9 can be translated in different reading frames in different bnAbs yet still target the same HA stem pocket. Thus, the D3-9 gene segment in the human immune repertoire can provide a robust defense against influenza virus. Elsevier Inc. 2018-10-10 2018-10-10 /pmc/articles/PMC6327842/ /pubmed/30308159 http://dx.doi.org/10.1016/j.chom.2018.09.010 Text en © 2018 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Wu, Nicholas C. Yamayoshi, Seiya Ito, Mutsumi Uraki, Ryuta Kawaoka, Yoshihiro Wilson, Ian A. Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene |
title | Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene |
title_full | Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene |
title_fullStr | Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene |
title_full_unstemmed | Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene |
title_short | Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene |
title_sort | recurring and adaptable binding motifs in broadly neutralizing antibodies to influenza virus are encoded on the d3-9 segment of the ig gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327842/ https://www.ncbi.nlm.nih.gov/pubmed/30308159 http://dx.doi.org/10.1016/j.chom.2018.09.010 |
work_keys_str_mv | AT wunicholasc recurringandadaptablebindingmotifsinbroadlyneutralizingantibodiestoinfluenzavirusareencodedonthed39segmentoftheiggene AT yamayoshiseiya recurringandadaptablebindingmotifsinbroadlyneutralizingantibodiestoinfluenzavirusareencodedonthed39segmentoftheiggene AT itomutsumi recurringandadaptablebindingmotifsinbroadlyneutralizingantibodiestoinfluenzavirusareencodedonthed39segmentoftheiggene AT urakiryuta recurringandadaptablebindingmotifsinbroadlyneutralizingantibodiestoinfluenzavirusareencodedonthed39segmentoftheiggene AT kawaokayoshihiro recurringandadaptablebindingmotifsinbroadlyneutralizingantibodiestoinfluenzavirusareencodedonthed39segmentoftheiggene AT wilsoniana recurringandadaptablebindingmotifsinbroadlyneutralizingantibodiestoinfluenzavirusareencodedonthed39segmentoftheiggene |