Mutational Analysis of the Role of the Glucansucrase Gtf180-ΔN Active Site Residues in Product and Linkage Specificity with Lactose as Acceptor Substrate

[Image: see text] Glucansucrase Gtf180-ΔN from Lactobacillus reuteri uses lactose as acceptor substrate to synthesize five glucosylated lactose molecules (F1–F5) with a degree of polymerization (DP) of 3–4 (GL34) and with (α1→2)/(α1→3)/(α1→4) glycosidic linkages. Q1140/W1065/N1029 mutations signific...

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Detalles Bibliográficos
Autores principales: Pham, Hien, Pijning, Tjaard, Dijkhuizen, Lubbert, van Leeuwen, Sander S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328278/
https://www.ncbi.nlm.nih.gov/pubmed/30396274
http://dx.doi.org/10.1021/acs.jafc.8b04486

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328278/
https://www.ncbi.nlm.nih.gov/pubmed/30396274
http://dx.doi.org/10.1021/acs.jafc.8b04486

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