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Selection of reference genes for quantitative real-time PCR normalization in the plant pathogen Puccinia helianthi Schw.

BACKGROUND: Real-time RT-PCR has become a common and robust technique to detect and quantify low-abundance mRNA expression and is a prefered tool when examining fungal gene expression in infected host tissues. However, correct evaluation of gene expression data requires accurate and reliable normali...

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Detalles Bibliográficos
Autores principales: Song, Yang, Wang, Yan, Guo, Dandan, Jing, Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329156/
https://www.ncbi.nlm.nih.gov/pubmed/30634896
http://dx.doi.org/10.1186/s12870-019-1629-x