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Selection of reference genes for quantitative real-time PCR normalization in the plant pathogen Puccinia helianthi Schw.
BACKGROUND: Real-time RT-PCR has become a common and robust technique to detect and quantify low-abundance mRNA expression and is a prefered tool when examining fungal gene expression in infected host tissues. However, correct evaluation of gene expression data requires accurate and reliable normali...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329156/ https://www.ncbi.nlm.nih.gov/pubmed/30634896 http://dx.doi.org/10.1186/s12870-019-1629-x |