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Improved DNA extraction technique from clot for the diagnosis of Chagas disease

BACKGROUND: The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater sensitivity than either buffy coat or whole blood samples. However, phenol-chl...

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Detalles Bibliográficos
Autores principales: Mayta, Holger, Romero, Yomara K., Pando, Alejandra, Verastegui, Manuela, Tinajeros, Freddy, Bozo, Ricardo, Henderson-Frost, Josephine, Colanzi, Rony, Flores, Jorge, Lerner, Richard, Bern, Caryn, Gilman, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329489/
https://www.ncbi.nlm.nih.gov/pubmed/30633743
http://dx.doi.org/10.1371/journal.pntd.0007024