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Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy

Two-photon microscopy is used to image neuronal activity, but has severe limitations for studying deeper cortical layers. Here, we developed a custom three-photon microscope optimized to image a vertical column of the cerebral cortex > 1 mm in depth in awake mice with low (<20 mW) average lase...

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Detalles Bibliográficos
Autores principales: Yildirim, Murat, Sugihara, Hiroki, So, Peter T. C., Sur, Mriganka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329792/
https://www.ncbi.nlm.nih.gov/pubmed/30635577
http://dx.doi.org/10.1038/s41467-018-08179-6