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Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy
Two-photon microscopy is used to image neuronal activity, but has severe limitations for studying deeper cortical layers. Here, we developed a custom three-photon microscope optimized to image a vertical column of the cerebral cortex > 1 mm in depth in awake mice with low (<20 mW) average lase...
Autores principales: | Yildirim, Murat, Sugihara, Hiroki, So, Peter T. C., Sur, Mriganka |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329792/ https://www.ncbi.nlm.nih.gov/pubmed/30635577 http://dx.doi.org/10.1038/s41467-018-08179-6 |
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