Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

BACKGROUND: The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs). However, recent advancement of biotechnology has shown substantial recovery of bio...

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Detalles Bibliográficos
Autores principales: Sarker, Animesh, Rathore, Abhishek Singh, Gupta, Rinkoo Devi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330739/
https://www.ncbi.nlm.nih.gov/pubmed/30642336
http://dx.doi.org/10.1186/s12934-019-1053-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330739/
https://www.ncbi.nlm.nih.gov/pubmed/30642336
http://dx.doi.org/10.1186/s12934-019-1053-9

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