Holistic engineering of Cal-A lipase chain-length selectivity identifies triglyceride binding hot-spot

Through the application of a region-focused saturation mutagenesis and randomization approach, protein engineering of the Cal-A enzyme was undertaken with the goal of conferring new triglyceride selectivity. Little is known about the mode of triglyceride binding to Cal-A. Engineering Cal-A thus requ...

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Detalles Bibliográficos
Autores principales: Quaglia, Daniela, Alejaldre, Lorea, Ouadhi, Sara, Rousseau, Olivier, Pelletier, Joelle N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331120/
https://www.ncbi.nlm.nih.gov/pubmed/30640952
http://dx.doi.org/10.1371/journal.pone.0210100

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331120/
https://www.ncbi.nlm.nih.gov/pubmed/30640952
http://dx.doi.org/10.1371/journal.pone.0210100

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