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Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

BACKGROUND: De novo DNA methylation triggered by short interfering RNAs is called RNA-directed DNA methylation (RdDM). Transcriptional gene silencing (TGS) through RdDM can be induced using a viral vector. We have previously induced RdDM on the 35S promoter in the green fluorescent protein (GFP)-exp...

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Detalles Bibliográficos
Autores principales: Matsunaga, Wataru, Shimura, Hanako, Shirakawa, Senri, Isoda, Reika, Inukai, Tsuyoshi, Matsumura, Takeshi, Masuta, Chikara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332629/
https://www.ncbi.nlm.nih.gov/pubmed/30642254
http://dx.doi.org/10.1186/s12870-019-1628-y