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Quantitative identification of proteins that influence miRNA biogenesis by RNA pull-down–SILAC mass spectrometry (RP–SMS)
RNA-binding proteins mediate and control gene expression. As some examples, they regulate pre-mRNA synthesis and processing; mRNA localisation, translation and decay; and microRNA (miRNA) biogenesis and function. Here, we present a detailed protocol for RNA pull-down coupled to stable isotope labell...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Academic Press
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335501/ https://www.ncbi.nlm.nih.gov/pubmed/29890283 http://dx.doi.org/10.1016/j.ymeth.2018.06.006 |