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Optimization of cell culture and cell disruption processes to enhance the production of thermophilic cellulase FnCel5A in E.coli using response surface methodology

FnCel5A from Fervidobacterium nodosum is one of the most thermostable endoglucanases that have phenomenal characteristics, such as high activity, pH stability, and multi-specificity towards various substrates. However, large-scale thermophilic enzyme production is still a challenge. Herein, we focus...

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Detalles Bibliográficos
Autores principales: Mohammad, Shah Faisal, Feng, Yan, Yang, Guangyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336418/
https://www.ncbi.nlm.nih.gov/pubmed/30653549
http://dx.doi.org/10.1371/journal.pone.0210595