CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection

CRISPR/Cas9 causes double-stranded DNA breaks that can undergo DNA repair either via non-homologous end joining (NHEJ) or, in the presence of a template, homology-directed repair (HDR). HDR is typically used to insert a specific genetic modification into the genome but has low efficiency compared to...

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Detalles Bibliográficos
Autores principales: Supharattanasitthi, Wasu, Carlsson, Emil, Sharif, Umar, Paraoan, Luminita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336765/
https://www.ncbi.nlm.nih.gov/pubmed/30655567
http://dx.doi.org/10.1038/s41598-018-36740-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336765/
https://www.ncbi.nlm.nih.gov/pubmed/30655567
http://dx.doi.org/10.1038/s41598-018-36740-2

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