Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification

Interaction between dietary flavonoids and albumins plays an important role in the bioavailability and bioactivity of flavonoids. Therefore, the influence of this interaction on the antioxidant activity of flavonoid has attracted much interest. In this study, a ceric reducing/antioxidant capacity as...

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Detalles Bibliográficos
Autores principales: Geng, Rui, Ma, Lei, Liu, Liangliang, Xie, Yixi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337117/
https://www.ncbi.nlm.nih.gov/pubmed/30585235
http://dx.doi.org/10.3390/molecules24010070
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author Geng, Rui
Ma, Lei
Liu, Liangliang
Xie, Yixi
author_facet Geng, Rui
Ma, Lei
Liu, Liangliang
Xie, Yixi
author_sort Geng, Rui
collection PubMed
description Interaction between dietary flavonoids and albumins plays an important role in the bioavailability and bioactivity of flavonoids. Therefore, the influence of this interaction on the antioxidant activity of flavonoid has attracted much interest. In this study, a ceric reducing/antioxidant capacity assay (CRAC) was employed to investigate the effects of albumin-flavonoid interaction on the antioxidant activity of seven common flavonoids. The results obtained from the CRAC assay were also compared separately with the results from the spectrophotometric methods including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. All the flavonoids show a decreasing in the antioxidant activity detected by CRAC assay, indicting a “masking effect” of bovine serum albumin (BSA)-flavonoid interaction. However, the results from DPPH and FRAP assays were conflicting, which may be attributed to the influence of solvent systems.
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spelling pubmed-63371172019-01-25 Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification Geng, Rui Ma, Lei Liu, Liangliang Xie, Yixi Molecules Article Interaction between dietary flavonoids and albumins plays an important role in the bioavailability and bioactivity of flavonoids. Therefore, the influence of this interaction on the antioxidant activity of flavonoid has attracted much interest. In this study, a ceric reducing/antioxidant capacity assay (CRAC) was employed to investigate the effects of albumin-flavonoid interaction on the antioxidant activity of seven common flavonoids. The results obtained from the CRAC assay were also compared separately with the results from the spectrophotometric methods including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. All the flavonoids show a decreasing in the antioxidant activity detected by CRAC assay, indicting a “masking effect” of bovine serum albumin (BSA)-flavonoid interaction. However, the results from DPPH and FRAP assays were conflicting, which may be attributed to the influence of solvent systems. MDPI 2018-12-25 /pmc/articles/PMC6337117/ /pubmed/30585235 http://dx.doi.org/10.3390/molecules24010070 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Geng, Rui
Ma, Lei
Liu, Liangliang
Xie, Yixi
Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification
title Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification
title_full Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification
title_fullStr Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification
title_full_unstemmed Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification
title_short Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification
title_sort influence of bovine serum albumin-flavonoid interaction on the antioxidant activity of dietary flavonoids: new evidence from electrochemical quantification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337117/
https://www.ncbi.nlm.nih.gov/pubmed/30585235
http://dx.doi.org/10.3390/molecules24010070
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