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Rapid Identification of Functional Pyrrolysyl-tRNA Synthetases via Fluorescence-Activated Cell Sorting

The orthogonal pyrrolysyl-tRNA synthetase/tRNA(CUA) pair and their variants have provided powerful tools for expanding the genetic code to allow for engineering of proteins with augmented structure and function not present in Nature. To expedite the discovery of novel pyrrolysyl-tRNA synthetase (Pyl...

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Detalles Bibliográficos
Autores principales: Lin, Andrew E., Lin, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337664/
https://www.ncbi.nlm.nih.gov/pubmed/30577609
http://dx.doi.org/10.3390/ijms20010029