Robust and stable transcriptional repression in Giardia using CRISPRi

Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of nonhomologous end joining and markers for positive selection, has stalled the adaptation of CRISPR/Cas9-mediated genetic tools for...

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Detalles Bibliográficos
Autores principales: McInally, S. G., Hagen, K. D., Nosala, C., Williams, J., Nguyen, K., Booker, J., Jones, K., Dawson, Scott C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337905/
https://www.ncbi.nlm.nih.gov/pubmed/30379614
http://dx.doi.org/10.1091/mbc.E18-09-0605

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337905/
https://www.ncbi.nlm.nih.gov/pubmed/30379614
http://dx.doi.org/10.1091/mbc.E18-09-0605

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