Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

A major challenge in genomics is the knowledge gap between sequence and its encoded function. Gain-of-function methods based on gene overexpression are attractive avenues for phenotype-based functional screens, but are not easily applied in high-throughput across many experimental conditions. Here,...

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Detalles Bibliográficos
Autores principales: Mutalik, Vivek K., Novichkov, Pavel S., Price, Morgan N., Owens, Trenton K., Callaghan, Mark, Carim, Sean, Deutschbauer, Adam M., Arkin, Adam P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338753/
https://www.ncbi.nlm.nih.gov/pubmed/30659179
http://dx.doi.org/10.1038/s41467-018-08177-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338753/
https://www.ncbi.nlm.nih.gov/pubmed/30659179
http://dx.doi.org/10.1038/s41467-018-08177-8

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